432 IL-33 is a negative regulator of filaggrin expression in the skin but not in barrier homeostasis

The skin protects the human body by providing barrier function. Interleukin (IL)-33 is a tissue-derived cytokine, mainly expressed epithelial and endothelial cells, known to activate Th2 lymphocytes, mast ILC2, eosinophils. We aimed study role of IL-33 in Wild-type (WT), knockout (IL-33KO) mice were tape stripped, trans-epidermal water loss (TEWL) was monitored. Skin samples corrected, hematoxylin eosin staining other immunohistochemical analyses performed. Total RNA extracted from real-time PCR Tape stripping increased TEWL both IL-33KO WT (p<0.0001), but there no statistical difference between (n.s.). thickness epidermis granular layer compared mice. observed an level filaggrin (FLG) at days 1, 2, 3 after tape-stripping IL-33KO, showed expression before day1 (p<0.0001) mice, reduced tape-stripping. Increased mRNA TSLP without day2 which Tape-stripping induced acid ceramidase (ASAH1 ASAH1.2) analysis not These results imply that protein existence did contribute disruption inhibition Expression with absence neither contributed stripping. There would be some molecules involved